Hotspot

ndhF

SSC · 108083-110317 · 2235 bp

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Pi 0.467

MarkerSeek score 80.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 920
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	CGAAAGAGGTAAGATCCGTTGA	55.0	45.5	57.9	57.9	2594 bp	0.5	42.0
ndhF_p2	CTAGCACTGGGTAGACCTCA	TCGAAAGAGGTAAGATCCGTTG	55.0	45.5	57.9	57.9	2595 bp	0.5	42.0
ndhF_p3	ATCAATTCGGTCGTTGTGGT	CGAAAGAGGTAAGATCCGTTGA	45.0	45.5	57.8	57.9	2792 bp	0.5	41.7
ndhF_p4	ATCAATTCGGTCGTTGTGGT	TCGAAAGAGGTAAGATCCGTTG	45.0	45.5	57.8	57.9	2793 bp	0.5	41.7
ndhF_p5	TCTCTTCCTTCTCCGAGCTC	CGAAAGAGGTAAGATCCGTTGA	55.0	45.5	58.2	57.9	2724 bp	0.5	41.2