Hotspot

ndhD

SSC · 113448-114950 · 1503 bp

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Pi 0.485

MarkerSeek score 80.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 609
pi: 0.485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAGTGATTCACGCGGTTCT	ATTTTGGAGCACGGGTTTCT	50.0	45.0	58.0	58.0	1714 bp	0.5	59.9
ndhD_p2	GTAGTGATTCACGCGGTTCT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	1912 bp	0.5	59.8
ndhD_p3	GTAGTGATTCACGCGGTTCT	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	2132 bp	0.5	59.2
ndhD_p4	GTAGTGATTCACGCGGTTCT	GGGTTTCTCTGGTCCAAGTG	50.0	55.0	58.0	58.1	1702 bp	0.5	59.1
ndhD_p5	GTAGTGATTCACGCGGTTCT	TGATACCTTGGGACGGATGT	50.0	50.0	58.0	58.1	1950 bp	0.5	58.9