Hotspot

ndhA

SSC · 117535-119672 · 2138 bp

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Pi 0.471

MarkerSeek score 80.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2138
variable_sites: 886
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCAATATACCTTGCGGC	GTCGCTGCTGATTTACCTCA	50.0	50.0	58.0	58.0	2984 bp	0.5	59.6
ndhA_p2	TGACCAATATACCTTGCGGC	TCAACAGGCCCTAGAAGGAA	50.0	50.0	58.0	58.0	2646 bp	0.5	59.4
ndhA_p3	TGACCAATATACCTTGCGGC	TTCAACAGGCCCTAGAAGGA	50.0	50.0	58.0	58.0	2647 bp	0.5	59.4
ndhA_p4	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	50.0	50.0	58.0	58.1	2719 bp	0.5	59.2
ndhA_p5	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	50.0	50.0	58.0	58.1	2720 bp	0.5	59.2