Hotspot

psaC-ndhE

SSC · 115316-115549 · 234 bp

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Pi 0.449

MarkerSeek score 75.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 234
variable_sites: 102
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGAAACCCGTGCTCCAAAAT	TTAATCGGGGCAATTGCTGT	45.0	45.0	58.0	58.2	1129 bp	0.5	58.7
psaC-ndhE_p2	TCCTCTGTTCTTGGAGCAGA	TTAATCGGGGCAATTGCTGT	50.0	45.0	58.0	58.2	931 bp	0.5	58.6
psaC-ndhE_p3	AGAAACCCGTGCTCCAAAAT	GTTAATCGGGGCAATTGCTG	45.0	50.0	58.0	57.8	1130 bp	0.5	58.3
psaC-ndhE_p4	TCCTCTGTTCTTGGAGCAGA	GTTAATCGGGGCAATTGCTG	50.0	50.0	58.0	57.8	932 bp	0.5	58.2
psaC-ndhE_p5	CGGACACTCAAGAAATCCGT	TTAATCGGGGCAATTGCTGT	50.0	45.0	57.9	58.2	988 bp	0.5	58.1