Hotspot

rpl32-trnL-UAG

SSC · 111521-112048 · 528 bp

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Pi 0.44

MarkerSeek score 78.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 528
variable_sites: 187
pi: 0.44
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAAGGATATTGGTCGGCGT	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.1	57.9	1267 bp	0.5	58.8
rpl32-trnL-UAG_p2	GAAAGGATATTGGTCGGCGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	667 bp	0.5	58.6
rpl32-trnL-UAG_p3	GAAAGGATATTGGTCGGCGT	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.1	57.8	660 bp	0.5	58.1
rpl32-trnL-UAG_p4	GAAAGGATATTGGTCGGCGT	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.1	57.8	666 bp	0.5	58.0
rpl32-trnL-UAG_p5	GAAAGGATATTGGTCGGCGT	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.1	57.8	665 bp	0.5	58.0