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ndhF-rpl32

SSC · 110318-111355 · 1038 bp

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Pi 0.429

MarkerSeek score 79.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1038
variable_sites: 354
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGGGGAAATTGTGCGGATT	ACGCCGACCAATATCCTTTC	45.0	50.0	58.1	58.1	1861 bp	0.5	59.1
ndhF-rpl32_p2	AGGGGAAATTGTGCGGATTT	ACGCCGACCAATATCCTTTC	45.0	50.0	58.1	58.1	1860 bp	0.5	59.1
ndhF-rpl32_p3	GGGAAATTGTGCGGATTTCG	ACGCCGACCAATATCCTTTC	50.0	50.0	58.1	58.1	1858 bp	0.5	58.9
ndhF-rpl32_p4	AACAGGTCGTGTGAACCAAA	ACGCCGACCAATATCCTTTC	45.0	50.0	57.9	58.1	1633 bp	0.5	58.7
ndhF-rpl32_p5	ACAGGTCGTGTGAACCAAAA	ACGCCGACCAATATCCTTTC	45.0	50.0	57.9	58.1	1632 bp	0.5	58.7