Hotspot

rps16

LSC · 4494-5596 · 1103 bp

Back to Leersia

Pi 0.00272

MarkerSeek score 60.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1103
variable_sites: 3
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACAAGGGATACTAGTGAGCCT	CCATAGATCGCAAAGCAAGT	47.6	45.0	57.6	56.2	1280 bp	1.0	54.6
rps16_p2	ACAAGGGATACTAGTGAGCCT	CCATAGATCGCAAAGCAAGTT	47.6	42.9	57.6	57.0	1280 bp	1.0	52.6
rps16_p3	GGGATACTAGTGAGCCTTTCC	CCATAGATCGCAAAGCAAGT	52.4	45.0	57.2	56.2	1276 bp	1.0	51.8
rps16_p4	ACAAGGGATACTAGTGAGCCT	TCCCTGTAACACTTTGCAATGA	47.6	40.9	57.6	58.2	1197 bp	1.0	51.6
rps16_p5	ACAAGGGATACTAGTGAGCCT	CCCTGTAACACTTTGCAATGA	47.6	42.9	57.6	56.7	1196 bp	1.0	50.7