Hotspot

rps11-rpl36

LSC · 76615-76746 · 132 bp

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Pi 0.0152

MarkerSeek score 61.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 132
variable_sites: 2
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TCTACGTGCACTCTTCCGTA	CGAAGCATAAGCAAAGGCAG	50.0	50.0	58.2	57.8	254 bp	1.0	77.7
rps11-rpl36_p2	ACTCTCCTACGTGAACCCAT	CGAAGCATAAGCAAAGGCAG	50.0	50.0	57.8	57.8	199 bp	1.0	77.5
rps11-rpl36_p3	CGTGCACTCTTCCGTAAACT	CGAAGCATAAGCAAAGGCAG	50.0	50.0	58.2	57.8	250 bp	1.0	77.4
rps11-rpl36_p4	TCTACGTGCACTCTTCCGTA	GCGTGGACGAATTAGAGTCA	50.0	50.0	58.2	57.7	286 bp	1.0	76.9
rps11-rpl36_p5	ATTCTACGTGCACTCTTCCG	CGAAGCATAAGCAAAGGCAG	50.0	50.0	57.7	57.8	256 bp	1.0	76.9