Hotspot

atpI-atpH

LSC · 32003-32803 · 801 bp

Back to Leersia

Pi 0.00375

MarkerSeek score 60.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 3
pi: 0.00375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CGAATCCATGGAGGGTCATC	AGCCAATCCAGCAGCAATAA	55.0	45.0	57.8	57.9	874 bp	1.0	77.7
atpI-atpH_p2	CGAATCCATGGAGGGTCATC	GAAGCAAGACCTACAGCCAA	55.0	50.0	57.8	57.8	888 bp	1.0	77.4
atpI-atpH_p3	CGAATCCATGGAGGGTCATC	GCAGTACCTTGACCAACTCC	55.0	55.0	57.8	58.2	918 bp	1.0	77.3
atpI-atpH_p4	CGAATCCATGGAGGGTCATC	AAGCAGCAGCAATTAGTGGA	55.0	45.0	57.8	57.8	851 bp	1.0	77.2
atpI-atpH_p5	CGAATCCATGGAGGGTCATC	GCCAATCCAGCAGCAATAAC	55.0	50.0	57.8	57.8	873 bp	1.0	77.2