Hotspot

trnC-GCA-rpoB

LSC · 18920-20022 · 1103 bp

Back to Leersia

Pi 0.00272

MarkerSeek score 60.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 3
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	TGTCGAGTTGGGTCAGTACT	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.0	57.6	1323 bp	1.0	77.1
trnC-GCA-rpoB_p2	TGTCGAGTTGGGTCAGTACT	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.0	58.4	1322 bp	1.0	77.0
trnC-GCA-rpoB_p3	TGTCGAGTTGGGTCAGTACT	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.0	58.4	1324 bp	1.0	77.0
trnC-GCA-rpoB_p4	TGTCGAGTTGGGTCAGTACT	GTTCTTCTGCCAAGCCTTGA	50.0	50.0	58.0	58.4	1321 bp	1.0	77.0
trnC-GCA-rpoB_p5	TGTCGAGTTGGGTCAGTACT	TCAAGTTCTTCTGCCAAGCC	50.0	50.0	58.0	58.4	1325 bp	1.0	77.0