Hotspot

petN-trnC-GCA

LSC · 17964-18848 · 885 bp

Back to Leersia

Pi 0.00452

MarkerSeek score 61.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 4
pi: 0.00452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	TTTATTTTTGGCGGCATGGC	50.0	45.0	58.2	58.3	992-993 bp	1.0	77.0
petN-trnC-GCA_p2	CCACTCCTCCCCCATACTAC	TTTATTTTTGGCGGCATGGC	60.0	45.0	58.3	58.3	1048-1049 bp	1.0	76.0
petN-trnC-GCA_p3	TCCACTCCTCCCCCATACTA	TTTATTTTTGGCGGCATGGC	55.0	45.0	58.4	58.3	1049-1050 bp	1.0	75.3
petN-trnC-GCA_p4	CACTCCTCCCCCATACTACT	TTTATTTTTGGCGGCATGGC	55.0	45.0	57.2	58.3	1047-1048 bp	1.0	72.0
petN-trnC-GCA_p5	GCCCAAGCGAGACTTACTAT	TTTATTTTTGGCGGCATGGC	50.0	45.0	57.1	58.3	991-992 bp	1.0	71.1