Hotspot

rrn4.5-rrn5

SSC · 49332-49514 · 183 bp

Back to Lecanorchis

Pi 0.0575
MarkerSeek score 76.80
Primer available yes
Label rrn4.5-rrn5

Feature Metadata

label_name
rrn4.5-rrn5
feature_type
igs
region
SSC
length_bp
183
variable_sites
10
pi
0.0575
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn4.5-rrn5_p1 TGCAGTTGAGGCATCCTAAT CCTCTATGCCTAGGACACCA 45.0 55.0 56.9 57.9 246-265 bp 1.0 76.4
rrn4.5-rrn5_p2 TGCAGTTGAGGCATCCTAAT AAGTTCGGGATGGATTGGTG 45.0 50.0 56.9 57.9 271-290 bp 1.0 75.5
rrn4.5-rrn5_p3 TGCAGTTGAGGCATCCTAAT ACCAAGTTCGGGATGGATTG 45.0 50.0 56.9 57.9 274-293 bp 1.0 75.5
rrn4.5-rrn5_p4 TGCAGTTGAGGCATCCTAAT CAAGTTCGGGATGGATTGGT 45.0 50.0 56.9 57.9 272-291 bp 1.0 75.5
rrn4.5-rrn5_p5 TGCAGTTGAGGCATCCTAAT CACCAAGTTCGGGATGGATT 45.0 50.0 56.9 57.9 275-294 bp 1.0 75.4