Hotspot

rpl14-rpl16

LSC · 22314-22682 · 369 bp

Back to Lecanorchis

Pi 0.128

MarkerSeek score 78.60

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 43
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CAACTCTTTAGCTCCGCTGT	ATACGCTCTTCAGGCACTTG	50.0	50.0	57.9	58.0	737-754 bp	1.0	87.8
rpl14-rpl16_p2	GGGTTTCCTGCTTGATCGAT	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	958-975 bp	1.0	86.2
rpl14-rpl16_p3	GGGTTTCCTGCTTGATCGAT	CCTGCTTGGATCACTGCTAG	50.0	55.0	58.0	58.0	936-953 bp	1.0	86.1
rpl14-rpl16_p4	ACTTCGGGCGCTAATGAAAT	ATACGCTCTTCAGGCACTTG	45.0	50.0	58.0	58.0	1042-1059 bp	1.0	85.7
rpl14-rpl16_p5	ACTTCGGGCGCTAATGAAAT	CCTGCTTGGATCACTGCTAG	45.0	55.0	58.0	58.0	1020-1037 bp	1.0	85.6