Hotspot

accD-rps18

LSC · 11358-14676 · 3319 bp

Back to Lecanorchis

Pi 0.0563

MarkerSeek score 71.40

Primer available yes

Label accD-rps18

Feature Metadata

label_name: accD-rps18
feature_type: igs
region: LSC
length_bp: 3319
variable_sites: 161
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rps18_p1	AAATACAGGATCGCGGAACC	ATGCGCAGTTACGAAGGATT	50.0	45.0	58.1	58.0	1243-1508 bp	1.0	91.1
accD-rps18_p2	AAATACAGGATCGCGGAACC	CCCCGACCCCCTATAAGTAG	50.0	60.0	58.1	58.1	891-1174 bp	1.0	91.1
accD-rps18_p3	AAATACAGGATCGCGGAACC	CCCGACCCCCTATAAGTAGG	50.0	60.0	58.1	58.1	890-1173 bp	1.0	91.1
accD-rps18_p4	AAATACAGGATCGCGGAACC	CGATGTCGTAGGTTCGAGTC	50.0	55.0	58.1	57.9	1044-1315 bp	1.0	90.9
accD-rps18_p5	AAATACAGGATCGCGGAACC	CAAAAAGCCCCTTGAGGTCT	50.0	50.0	58.1	58.0	1441 bp	0.5	59.6