Hotspot

ycf1

SSC · 121662-127142 · 5481 bp

Back to Lavandula

Pi 0.0228

MarkerSeek score 53.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 230
pi: 0.0228
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAGGCCTCAATATCGGCTTT	AGTTCGCCAGCGTTGTATAG	50.0	50.0	58.0	58.1	402-414 bp	1.0	87.1
ycf1_p2	AAATTCGATATGGGGCGCTT	AGTTCGCCAGCGTTGTATAG	45.0	50.0	58.0	58.1	597 bp	0.75	71.0
ycf1_p3	AATTCGATATGGGGCGCTTT	AGTTCGCCAGCGTTGTATAG	45.0	50.0	58.0	58.1	596 bp	0.75	71.0
ycf1_p4	AAATTCGATATGGGGCGCTT	GAGTTCGCCAGCGTTGTATA	45.0	50.0	58.0	58.1	598 bp	0.75	70.9
ycf1_p5	AATTCGATATGGGGCGCTTT	GAGTTCGCCAGCGTTGTATA	45.0	50.0	58.0	58.1	597 bp	0.75	70.9