Hotspot

rpl32-trnL-UAG

SSC · 111318-112088 · 771 bp

Back to Lavandula

Pi 0.0425

MarkerSeek score 77.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 771
variable_sites: 57
pi: 0.0425
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCCCACATACGACGAAGATT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1408-1567 bp	1.0	88.6
rpl32-trnL-UAG_p2	GCCCACATACGACGAAGATT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1409-1568 bp	1.0	88.6
rpl32-trnL-UAG_p3	GCCCACATACGACGAAGATT	TCATGCCCTTTTCGGATGAG	50.0	50.0	58.1	58.0	1691-1844 bp	1.0	87.7
rpl32-trnL-UAG_p4	GCCCACATACGACGAAGATT	ATGCCCTTTTCGGATGAGTC	50.0	50.0	58.1	58.0	1689-1842 bp	1.0	87.7
rpl32-trnL-UAG_p5	GCCCACATACGACGAAGATT	GCCCTTTTCGGATGAGTCAT	50.0	50.0	58.1	58.0	1687-1840 bp	1.0	87.7