Hotspot

ndhF-rpl32

SSC · 110707-111146 · 440 bp

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Pi 0.0394

MarkerSeek score 72.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 440
variable_sites: 31
pi: 0.0394
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	TGCATGAATTGATGGCGAGA	50.0	45.0	58.1	58.0	1117-1154 bp	1.0	86.3
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	TGAATTGATGGCGAGAGGTG	50.0	50.0	58.1	58.0	1113-1150 bp	0.75	72.4
ndhF-rpl32_p3	GCCCACATACGACGAAGATT	GAATTGATGGCGAGAGGTGT	50.0	50.0	58.1	58.0	1112-1149 bp	0.75	72.4
ndhF-rpl32_p4	AAAAGCCCACATACGACGAA	TGAATTGATGGCGAGAGGTG	45.0	50.0	57.8	58.0	1117-1154 bp	0.75	71.5
ndhF-rpl32_p5	GCCCACATACGACGAAGATT	ACTCCCCCTCTGGAAAGAAA	50.0	50.0	58.1	57.9	980-999 bp	0.5	63.3