Hotspot

rps8-rpl14

LSC · 79404-79584 · 181 bp

Back to Lavandula

Pi 0.0259

MarkerSeek score 69.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 8
pi: 0.0259
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATGCTATGCGAACAACTCT	CTCGCATTTTTGGGGCAATC	42.9	50.0	57.5	58.4	343-355 bp	1.0	67.9
rps8-rpl14_p2	AGATGCTATGCGAACAACTCT	AGGAACTCGCATTTTTGGGG	42.9	50.0	57.5	58.7	348-360 bp	1.0	64.7
rps8-rpl14_p3	AGATGCTATGCGAACAACTC	CTCGCATTTTTGGGGCAATC	45.0	50.0	55.9	58.4	343-355 bp	1.0	63.7
rps8-rpl14_p4	GATGCTATGCGAACAACTCT	CTCGCATTTTTGGGGCAATC	45.0	50.0	55.9	58.4	342-354 bp	1.0	63.7
rps8-rpl14_p5	AGATGCTATGCGAACAACTCT	CCCCGCGAATTGAGACAATT	42.9	50.0	57.5	58.9	323-335 bp	1.0	63.6