Hotspot

ndhC-trnV-UAC

LSC · 50069-51001 · 933 bp

Back to Lavandula

Pi 0.021
MarkerSeek score 68.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
933
variable_sites
36
pi
0.021
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 27.3 50.0 52.7 57.8 968-989 bp 1.0 46.9
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 27.3 55.0 52.7 57.5 967-988 bp 1.0 46.9
ndhC-trnV-UAC_p3 AGACTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 30.8 50.0 56.5 57.8 1001-1022 bp 1.0 46.8
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAAAAACA AAGGCATGTTGGGTCTTTGA 27.3 45.0 52.7 57.6 1035-1056 bp 1.0 46.7
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAAAAACA AGGCATGTTGGGTCTTTGAA 27.3 45.0 52.7 57.6 1034-1055 bp 1.0 46.7