Hotspot

trnF-GAA-ndhJ

LSC · 47698-48381 · 684 bp

Back to Lavandula

Pi 0.0346

MarkerSeek score 64.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 44
pi: 0.0346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	825-836 bp	1.0	84.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	789-800 bp	1.0	83.9
trnF-GAA-ndhJ_p3	GAGGGTGGGATGCTACATTG	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	854-865 bp	1.0	83.8
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	TGCCTGACAGTTGGATAGGA	55.0	50.0	58.0	58.0	834-845 bp	1.0	83.8
trnF-GAA-ndhJ_p5	GAGGGTGGGATGCTACATTG	TGCCTGACAGTTGGATAGGA	55.0	50.0	58.0	58.0	863-874 bp	1.0	83.4