Hotspot

rps16-trnQ-UUG

LSC · 5843-6733 · 891 bp

Back to Lavandula

Pi 0.0465
MarkerSeek score 76.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
891
variable_sites
74
pi
0.0465
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACAACGGGTCCTATCCTTCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 983-1070 bp 1.0 87.1
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 964-1051 bp 1.0 86.6
rps16-trnQ-UUG_p3 GGTCCTATCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.8 58.1 977-1064 bp 1.0 85.8
rps16-trnQ-UUG_p4 ACGGGTCCTATCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 980-1067 bp 1.0 85.6
rps16-trnQ-UUG_p5 CTATCCTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 973-1060 bp 1.0 85.4