Hotspot

ycf80

Genome · 56133-57560 · 1428 bp

Back to Laurencia

Pi 0.121

MarkerSeek score 78.80

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1428
variable_sites: 300
pi: 0.121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	ACTTTGGTGGTCGAAGGTTC	TTTCCACCTGGTGAACATGG	50.0	50.0	58.0	58.0	2527-2529 bp	1.0	87.7
ycf80_p2	GACTTTGGTGGTCGAAGGTT	TTTCCACCTGGTGAACATGG	50.0	50.0	58.0	58.0	2528-2530 bp	1.0	87.7
ycf80_p3	CTTTGGTGGTCGAAGGTTCA	TTTCCACCTGGTGAACATGG	50.0	50.0	58.0	58.0	2526-2528 bp	1.0	87.7
ycf80_p4	ACTTTGGTGGTCGAAGGTTC	TTAGCACCAACGATACCTGC	50.0	50.0	58.0	58.0	2314-2316 bp	1.0	87.6
ycf80_p5	GACTTTGGTGGTCGAAGGTT	TTAGCACCAACGATACCTGC	50.0	50.0	58.0	58.0	2315-2317 bp	1.0	87.6