Hotspot

ycf60

Genome · 27427-28023 · 597 bp

Back to Laurencia

Pi 0.0678

MarkerSeek score 80.80

Primer available yes

Label ycf60

Feature Metadata

label_name: ycf60
feature_type: gene
region: Genome
length_bp: 597
variable_sites: 74
pi: 0.0678
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf60_p1	TTGCGTATTGTACACGACGT	GAGTTGACCATTCGCCTGAT	45.0	50.0	58.0	58.0	1206 bp	0.75	77.2
ycf60_p2	CGATGTGCCGCTATCGATAA	GAGTTGACCATTCGCCTGAT	50.0	50.0	58.0	58.0	1169 bp	0.25	49.6
ycf60_p3	TAGTCCTTGACCTGAGCCAA	GAGTTGACCATTCGCCTGAT	50.0	50.0	58.0	58.0	1568 bp	0.25	49.6
ycf60_p4	CCTGAGCCAATAGGTGAACC	GAGTTGACCATTCGCCTGAT	55.0	50.0	58.0	58.0	1558 bp	0.25	49.4
ycf60_p5	TGACCTGAGCCAATAGGTGA	GAGTTGACCATTCGCCTGAT	50.0	50.0	58.0	58.0	1561 bp	0.25	49.4