Hotspot

ycf38

Genome · 137733-138605 · 873 bp

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Pi 0.0895

MarkerSeek score 79.40

Primer available yes

Label ycf38

Feature Metadata

label_name: ycf38
feature_type: gene
region: Genome
length_bp: 873
variable_sites: 135
pi: 0.0895
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf38_p1	CAGCACGACACGAATAAGGA	TCCCAGTATACCCAATGCCA	50.0	50.0	58.0	58.1	1819-1829 bp	0.75	78.3
ycf38_p2	CAGCACGACACGAATAAGGA	GTGATACTCCATCCTCCCCA	50.0	55.0	58.0	57.9	1706-1715 bp	0.5	67.8
ycf38_p3	CAGCACGACACGAATAAGGA	TGAATCCATCTGGTCCCCAT	50.0	50.0	58.0	58.1	2034-2044 bp	0.5	67.0
ycf38_p4	CAGCACGACACGAATAAGGA	AAGCTCCATAGACCGGGATT	50.0	50.0	58.0	58.2	1741-1751 bp	0.5	66.5
ycf38_p5	CAGCACGACACGAATAAGGA	CTTCGAAGCTCCATAGACCG	50.0	55.0	58.0	57.9	1756 bp	0.25	48.9