Hotspot

rnz

Genome · 18536-19213 · 678 bp

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Pi 0.0944

MarkerSeek score 79.40

Primer available yes

Label rnz

Feature Metadata

label_name: rnz
feature_type: gene
region: Genome
length_bp: 678
variable_sites: 116
pi: 0.0944
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rnz_p1	TAATTGCTGCTGGTTCCTGG	TGCTACCTCTCTGACGTACA	50.0	50.0	58.2	57.5	1656-1675 bp	0.5	64.0
rnz_p2	TAATTGCTGCTGGTTCCTGG	TCACGATACCGGAAACAAGC	50.0	50.0	58.2	58.3	1747 bp	0.25	46.4
rnz_p3	TAATTGCTGCTGGTTCCTGG	CGATACCGGAAACAAGCGAA	50.0	50.0	58.2	58.6	1744 bp	0.25	43.5
rnz_p4	AGTAATTGCTGCTGGTTCCT	TCACGATACCGGAAACAAGC	45.0	50.0	57.1	58.3	1749 bp	0.25	40.6
rnz_p5	TAATTGCTGCTGGTTCCTGG	CGATACCGGAAACAAGCGA	50.0	52.6	58.2	58.0	1744 bp	0.25	40.4