Hotspot

orf103

Genome · 165418-165918 · 501 bp

Back to Laurencia

Pi 0.159

MarkerSeek score 76.40

Primer available yes

Label orf103

Feature Metadata

label_name: orf103
feature_type: gene
region: Genome
length_bp: 501
variable_sites: 114
pi: 0.159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf103_p1	GCTGCCATTGAAGAGGGTAT	TGATGCGTGGGAACAATTGA	50.0	45.0	57.6	58.1	1138-1141 bp	0.5	64.4
orf103_p2	GCAACAAAAGCTGCCATTGA	TGATGCGTGGGAACAATTGA	45.0	45.0	58.1	58.1	1147 bp	0.25	48.2
orf103_p3	GCAACAAAAGCTGCCATTGA	AGAAATGATGCGTGGGAACA	45.0	45.0	58.1	57.8	1152 bp	0.25	47.4
orf103_p4	GCAACAAAAGCTGCCATTGA	TTTGGCCAAGAAATGATGCG	45.0	45.0	58.1	57.6	1160 bp	0.25	46.0
orf103_p5	TGCAACAAAAGCTGCCATTG	TGATGCGTGGGAACAATTGA	45.0	45.0	58.4	58.1	1148 bp	0.25	45.9