Hotspot

mat

Genome · 66475-67518 · 1044 bp

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Pi 0.169

MarkerSeek score 79.00

Primer available yes

Label mat

Feature Metadata

label_name: mat
feature_type: gene
region: Genome
length_bp: 1044
variable_sites: 300
pi: 0.169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
mat_p1	AACCAGCGGTGTCCTTTTAG	TCATGTGCGACCCGTTTTTA	50.0	45.0	58.1	58.1	1901-1908 bp	0.75	77.1
mat_p2	GAACCAGCGGTGTCCTTTTA	TCATGTGCGACCCGTTTTTA	50.0	45.0	58.1	58.1	1902-1909 bp	0.75	77.0
mat_p3	CTCAAGGTGCGGTTATGACA	TCATGTGCGACCCGTTTTTA	50.0	45.0	57.9	58.1	2450 bp	0.25	48.3
mat_p4	CCGCTACCCGCTTTATGAAT	TCATGTGCGACCCGTTTTTA	50.0	45.0	58.1	58.1	1960 bp	0.25	48.1
mat_p5	CTCAAGGTGCGGTTATGACA	CATGTGCGACCCGTTTTTAG	50.0	50.0	57.9	57.8	2449 bp	0.25	47.3