Hotspot

acsF

Genome · 109411-110460 · 1050 bp

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Pi 0.0753

MarkerSeek score 79.50

Primer available yes

Label acsF

Feature Metadata

label_name: acsF
feature_type: gene
region: Genome
length_bp: 1050
variable_sites: 138
pi: 0.0753
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acsF_p1	TCCCCAAACGACTAATGCAA	TTCGAACCCACGGAACTTTT	45.0	45.0	57.4	58.0	2053-2055 bp	0.5	63.0
acsF_p2	TTCCCCAAACGACTAATGCA	TTCGAACCCACGGAACTTTT	45.0	45.0	57.4	58.0	2054-2056 bp	0.5	63.0
acsF_p3	TCCCCAAACGACTAATGCAA	ACCAACTCTACCACCTCTCC	45.0	55.0	57.4	58.1	2001-2003 bp	0.5	63.0
acsF_p4	TTCCCCAAACGACTAATGCA	ACCAACTCTACCACCTCTCC	45.0	55.0	57.4	58.1	2002-2004 bp	0.5	63.0
acsF_p5	ACCATTTCTTCCCCAAACGA	TTCGAACCCACGGAACTTTT	45.0	45.0	57.3	58.0	2062 bp	0.25	44.3