Hotspot

ccsA-cpeA

Genome · 50967-51617 · 651 bp

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Pi 0.249

MarkerSeek score 81.70

Primer available yes

Label ccsA-cpeA

Feature Metadata

label_name: ccsA-cpeA
feature_type: igs
region: Genome
length_bp: 651
variable_sites: 244
pi: 0.249
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-cpeA_p1	ATGTCTTCTGGCAGGCAAAA	CCAACTGCTGCTTATGTTGC	45.0	50.0	58.0	58.0	1142 bp	0.25	49.9
ccsA-cpeA_p2	ATGTCTTCTGGCAGGCAAAA	AGGCGATTGTTCTGCACTAG	45.0	50.0	58.0	58.0	1632 bp	0.25	49.9
ccsA-cpeA_p3	ATGTCTTCTGGCAGGCAAAA	GAAGCAGGCGAGAATCAAGA	45.0	50.0	58.0	58.0	1298 bp	0.25	49.9
ccsA-cpeA_p4	ATGTCTTCTGGCAGGCAAAA	GAGAAGCAGGCGAGAATCAA	45.0	50.0	58.0	58.0	1300 bp	0.25	49.9
ccsA-cpeA_p5	ATGTCTTCTGGCAGGCAAAA	GCTGCTGTTAGCTAAGCTCA	45.0	50.0	58.0	58.0	1577 bp	0.25	49.9