Hotspot

ycf46-rpl34

Genome · 6303-6830 · 528 bp

Back to Laurencia

Pi 0.278

MarkerSeek score 82.30

Primer available yes

Label ycf46-rpl34

Feature Metadata

label_name: ycf46-rpl34
feature_type: igs
region: Genome
length_bp: 528
variable_sites: 233
pi: 0.278
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46-rpl34_p1	TTAAATTGCCTAAGCCGCCT	CGCTGAACAGGAAAAAGGGA	45.0	50.0	57.9	58.4	1714 bp	0.25	45.7
ycf46-rpl34_p2	TTAAATTGCCTAAGCCGCCT	TCGCTGAACAGGAAAAAGGG	45.0	50.0	57.9	58.4	1715 bp	0.25	45.7
ycf46-rpl34_p3	TTAAATTGCCTAAGCCGCCT	AGGATGTCGCAGGTTCAAAT	45.0	45.0	57.9	57.5	1980 bp	0.25	44.9
ycf46-rpl34_p4	TTAAATTGCCTAAGCCGCCT	ATGTCGCAGGTTCAAATCCT	45.0	45.0	57.9	57.5	1977 bp	0.25	44.9
ycf46-rpl34_p5	CCTAAGCCGCCTACATTGAT	CGCTGAACAGGAAAAAGGGA	50.0	50.0	57.4	58.4	1706 bp	0.25	42.4