Hotspot

trnL-rpl32

SSC · 124516-125442 · 927 bp

Back to Launaea

Pi 0.0221

MarkerSeek score 67.20

Primer available yes

Label trnL-rpl32

Feature Metadata

label_name: trnL-rpl32
feature_type: igs
region: SSC
length_bp: 927
variable_sites: 20
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-rpl32_p1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	50.0	50.0	58.0	57.6	1059-1079 bp	1.0	80.0
trnL-rpl32_p2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	50.0	50.0	58.0	57.6	1060-1080 bp	1.0	80.0
trnL-rpl32_p3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	1042-1062 bp	1.0	79.8
trnL-rpl32_p4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	50.0	45.0	58.0	57.6	1043-1063 bp	1.0	79.8
trnL-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	AAGGAAAGGATATTGGGCGG	50.0	50.0	57.9	57.6	1069-1089 bp	1.0	79.1