Hotspot

ycf1-rps15

SSC · 114864-115266 · 403 bp

Back to Launaea

Pi 0.0223

MarkerSeek score 64.60

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 403
variable_sites: 9
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ATGGAAGCCGCTTGAGTATG	AGTCTTCGTATCCTATTGGTGA	50.0	40.9	58.0	56.1	560-576 bp	1.0	49.7
ycf1-rps15_p2	GGAAGCCGCTTGAGTATGTT	AGTCTTCGTATCCTATTGGTGA	50.0	40.9	58.3	56.1	558-574 bp	1.0	48.0
ycf1-rps15_p3	ATGGAAGCCGCTTGAGTATG	AAGTCTTCGTATCCTATTGGTGA	50.0	39.1	58.0	56.8	561-577 bp	1.0	47.4
ycf1-rps15_p4	ATGGAAGCCGCTTGAGTATG	AGTCTTCGTATCCTATTGGTGAA	50.0	39.1	58.0	56.8	560-576 bp	1.0	47.4
ycf1-rps15_p5	GGAAGCCGCTTGAGTATGTT	AGTCTTCGTATCCTATTGGTGAA	50.0	39.1	58.3	56.8	558-574 bp	1.0	45.7