Hotspot

trnW-trnP

LSC · 67362-67523 · 162 bp

Back to Launaea

Pi 0.037
MarkerSeek score 65.10
Primer available yes
Label trnW-trnP

Feature Metadata

label_name
trnW-trnP
feature_type
igs
region
LSC
length_bp
162
variable_sites
6
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-trnP_p1 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 244-250 bp 1.0 80.9
trnW-trnP_p2 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 233-239 bp 1.0 80.9
trnW-trnP_p3 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 231-237 bp 1.0 80.9
trnW-trnP_p4 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 234-240 bp 1.0 80.9
trnW-trnP_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 245-251 bp 1.0 80.8