Hotspot

ycf4-cemA

LSC · 61218-62070 · 853 bp

Back to Launaea

Pi 0.0197

MarkerSeek score 60.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 16
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	995-1033 bp	1.0	83.9
ycf4-cemA_p2	GAGCCATTCCATTGACTCGT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1346-1384 bp	1.0	82.9
ycf4-cemA_p3	TCTTTTTATGGGATCGCGGG	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1596-1634 bp	1.0	82.4
ycf4-cemA_p4	TCTTGCGTGTACCGATTGAG	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1306 bp	0.5	59.5
ycf4-cemA_p5	TCTTGCGTGTACCGATTGAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	955 bp	0.5	59.3