Hotspot

ndhC-trnV

LSC · 50838-52097 · 1260 bp

Back to Launaea

Pi 0.0202

MarkerSeek score 63.80

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1260
variable_sites: 24
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TCCTTTGCTACTCGGAGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1389-1407 bp	1.0	83.4
ndhC-trnV_p2	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1380-1398 bp	1.0	82.0
ndhC-trnV_p3	TTCTCCGGTCCTTTGCTACT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	1397-1415 bp	1.0	81.6
ndhC-trnV_p4	TTTCTCCGGTCCTTTGCTAC	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.5	58.1	1398-1416 bp	1.0	80.8
ndhC-trnV_p5	CTCGGAGCTAAAAATCCCGA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1379-1397 bp	1.0	79.7