Hotspot

trnR-trnT

LSC · 30220-31478 · 1259 bp

Back to Launaea

Pi 0.0112

MarkerSeek score 66.60

Primer available yes

Label trnR-trnT

Feature Metadata

label_name: trnR-trnT
feature_type: igs
region: LSC
length_bp: 1259
variable_sites: 13
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnT_p1	AGAAGACCTCTGTCCTATCCA	ATTTGAACCGAGGGCTTACG	47.6	50.0	56.9	58.3	1258-1359 bp	1.0	66.3
trnR-trnT_p2	AGGTTTAGAAGACCTCTGTCCT	ATTTGAACCGAGGGCTTACG	45.5	50.0	57.9	58.3	1264-1365 bp	1.0	65.6
trnR-trnT_p3	AGAAGACCTCTGTCCTATCCA	GGATTTGAACCGAGGGCTTA	47.6	50.0	56.9	57.6	1260-1361 bp	1.0	65.1
trnR-trnT_p4	AGGTTTAGAAGACCTCTGTCCT	GGATTTGAACCGAGGGCTTA	45.5	50.0	57.9	57.6	1266-1367 bp	1.0	64.4
trnR-trnT_p5	AGAAGACCTCTGTCCTATCCA	ACGCCTTACCATGGGATTAC	47.6	50.0	56.9	57.4	1241-1342 bp	1.0	63.3