Hotspot

rps16

LSC · 4879-5995 · 1117 bp

Back to Latania

Pi 0.00179

MarkerSeek score 59.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1117
variable_sites: 2
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GTGAATACAGCAAGAAAAAGGCA	CGGTTGAGCCAACGACTATT	39.1	50.0	58.1	58.3	1297-1299 bp	1.0	52.9
rps16_p2	TGTGAATACAGCAAGAAAAAGGC	CGGTTGAGCCAACGACTATT	39.1	50.0	58.1	58.3	1298-1300 bp	1.0	52.9
rps16_p3	TGAATACAGCAAGAAAAAGGCA	CGGTTGAGCCAACGACTATT	36.4	50.0	56.8	58.3	1296-1298 bp	1.0	52.3
rps16_p4	GTGAATACAGCAAGAAAAAGGCA	TATACACACACCGGTTGAGC	39.1	50.0	58.1	57.6	1308-1310 bp	1.0	52.1
rps16_p5	TGTGAATACAGCAAGAAAAAGGC	TATACACACACCGGTTGAGC	39.1	50.0	58.1	57.6	1309-1311 bp	1.0	52.1