Hotspot

rpl16

LSC · 84708-86326 · 1619 bp

Back to Latania

Pi 0.00187

MarkerSeek score 60.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1619
variable_sites: 3
pi: 0.00187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAATGCAAAGGATGAAGGA	ACAAACTCTTTCATTCTTTTTCTGT	40.0	28.0	54.9	56.1	1814-1826 bp	1.0	40.7
rpl16_p2	TCAATGCAAAGGATGAAGGAA	ACAAACTCTTTCATTCTTTTTCTGT	38.1	28.0	55.7	56.1	1814-1826 bp	1.0	40.7
rpl16_p3	TTCAATGCAAAGGATGAAGGA	ACAAACTCTTTCATTCTTTTTCTGT	38.1	28.0	55.7	56.1	1815-1827 bp	1.0	40.7
rpl16_p4	TCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACT	40.0	28.0	54.9	55.8	1754-1766 bp	1.0	40.7
rpl16_p5	TCAATGCAAAGGATGAAGGA	ACAAACTCTTTCATTCTTTTTCTGTT	40.0	26.9	54.9	56.7	1814-1826 bp	1.0	40.7