Hotspot

psbC

LSC · 35533-36954 · 1422 bp

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Pi 0

MarkerSeek score 31.10

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTTCCCAAGAAATCCGTGCA	ATAGCCGAGCCAGAACAGAA	45.0	50.0	58.0	58.8	1586 bp	1.0	73.5
psbC_p2	TTTCCCAAGAAATCCGTGCA	TAGCCGAGCCAGAACAGAAA	45.0	50.0	58.0	59.0	1585 bp	1.0	71.7
psbC_p3	TTTCCCAAGAAATCCGTGCA	GCCGAGCCAGAACAGAAAAA	45.0	50.0	58.0	59.0	1583 bp	1.0	71.5
psbC_p4	TTTCCCAAGAAATCCGTGCA	ATAGCCGAGCCAGAACAGA	45.0	52.6	58.0	58.1	1586 bp	1.0	71.1
psbC_p5	GCAGCGGAAGATCCTGAATT	ATAGCCGAGCCAGAACAGAA	50.0	50.0	58.3	58.8	1569 bp	1.0	70.9