Hotspot

ccsA

SSC · 118625-119593 · 969 bp

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Pi 0

MarkerSeek score 30.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATCCTATAAA	ACTCGAACAAGCAAACTTTCG	50.0	42.9	57.5	57.7	1121 bp	1.0	65.1
ccsA_p2	GAGTGGCGGCATCCTATAAA	CTCGAACAAGCAAACTTTCGT	50.0	42.9	57.5	57.7	1120 bp	1.0	65.1
ccsA_p3	GAGTGGCGGCATCCTATAAA	TCGAACAAGCAAACTTTCGT	50.0	40.0	57.5	56.5	1119 bp	1.0	63.8
ccsA_p4	GAGTGGCGGCATCCTATAAA	TCGAACAAGCAAACTTTCGTT	50.0	38.1	57.5	57.2	1119 bp	1.0	61.6
ccsA_p5	AGTGGCGGCATCCTATAAAA	CTCGAACAAGCAAACTTTCGT	45.0	42.9	57.0	57.7	1119 bp	1.0	61.3