Hotspot

accD-psaI

LSC · 61845-62832 · 988 bp

Back to Latania

Pi 0.00102

MarkerSeek score 59.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1093-1098 bp	1.0	80.0
accD-psaI_p2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1094-1099 bp	1.0	80.0
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1099-1104 bp	1.0	79.4
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1100-1105 bp	1.0	79.4
accD-psaI_p5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1099-1104 bp	1.0	79.3