Hotspot

ndhC-trnV-UAC

LSC · 52440-54380 · 1941 bp

Back to Latania

Pi 0.0021

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1941
variable_sites: 4
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	2050-2085 bp	1.0	79.4
ndhC-trnV-UAC_p2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	2042-2077 bp	1.0	79.3
ndhC-trnV-UAC_p3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	50.0	45.0	58.1	58.5	2132-2167 bp	1.0	77.1
ndhC-trnV-UAC_p4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	2124-2159 bp	1.0	77.0
ndhC-trnV-UAC_p5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	2049-2084 bp	1.0	76.6