Hotspot

trnT-GGU-psbD

LSC · 33478-34523 · 1046 bp

Back to Latania

Pi 0.00192

MarkerSeek score 60.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 2
pi: 0.00192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1191-1196 bp	1.0	78.0
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1175-1180 bp	1.0	77.9
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1194-1199 bp	1.0	77.8
trnT-GGU-psbD_p4	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1210-1215 bp	1.0	77.7
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1178-1183 bp	1.0	77.7