Hotspot

petN-psbM

LSC · 30622-31354 · 733 bp

Back to Latania

Pi 0.00413

MarkerSeek score 61.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 3
pi: 0.00413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	889-901 bp	1.0	68.1
petN-psbM_p2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.7	57.6	884-896 bp	1.0	67.1
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	834-846 bp	1.0	66.8
petN-psbM_p4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.6	57.6	893-905 bp	1.0	65.1
petN-psbM_p5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.6	57.6	892-904 bp	1.0	64.4