Hotspot

ccsA-ndhD

SSC · 120514-120761 · 248 bp

Back to Lasianthus

Pi 0.0175

MarkerSeek score 65.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 248
variable_sites: 5
pi: 0.0175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTAGCGTCTATAGGC	TGCCCGTACTAGGTATTGGT	47.6	50.0	56.9	57.8	469-522 bp	1.0	64.0
ccsA-ndhD_p2	AGCAATTGTAGCGTCTATAGGC	TGCCCGTACTAGGTATTGGT	45.5	50.0	58.3	57.8	470-523 bp	1.0	61.7
ccsA-ndhD_p3	CCAGCAATTGTAGCGTCTAT	TGCCCGTACTAGGTATTGGT	45.0	50.0	55.6	57.8	472-525 bp	1.0	61.5
ccsA-ndhD_p4	TGGGTTTGTTATTTTGGGGT	TGCCCGTACTAGGTATTGGT	40.0	50.0	55.3	57.8	436-489 bp	1.0	58.9
ccsA-ndhD_p5	TGGGTTTGTTATTTTGGGGTT	TGCCCGTACTAGGTATTGGT	38.1	50.0	56.1	57.8	436-489 bp	1.0	57.5