Hotspot

petA-psbJ

LSC · 64502-65747 · 1246 bp

Back to Lasianthus

Pi 0.00645

MarkerSeek score 68.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 12
pi: 0.00645
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ACTGGTATTCTTGTGATCGGG	50.0	47.6	57.8	57.5	1341-1423 bp	1.0	71.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCTTGTGATCGGGT	50.0	47.6	57.8	57.5	1340-1422 bp	1.0	71.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	TGGTATTCTTGTGATCGGGT	50.0	45.0	57.8	56.2	1339-1421 bp	1.0	69.4
petA-psbJ_p4	TGTCCAAGGCCTTTTGTTCT	ACTGGTATTCTTGTGATCGGG	45.0	47.6	57.5	57.5	1348-1430 bp	1.0	68.7
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	CTGGTATTCTTGTGATCGGGT	45.0	47.6	57.5	57.5	1347-1429 bp	1.0	68.7