Hotspot

ndhC-trnV-UAC

LSC · 51999-52510 · 512 bp

Back to Lasianthus

Pi 0.0163

MarkerSeek score 71.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 12
pi: 0.0163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	564-652 bp	1.0	82.9
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	576-664 bp	1.0	81.2
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	563-651 bp	1.0	80.1
ndhC-trnV-UAC_p4	TAATCGGGGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	569-657 bp	1.0	79.1
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	552-640 bp	1.0	79.0