Hotspot

rps4-trnT-UGU

LSC · 46972-47540 · 569 bp

Back to Lasianthus

Pi 0.0234

MarkerSeek score 75.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 17
pi: 0.0234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CATTTCCGGCTCTAGGCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	672-723 bp	1.0	84.6
rps4-trnT-UGU_p2	GAACGCGATTGGTTTCGAAG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	694-745 bp	1.0	83.8
rps4-trnT-UGU_p3	CATTTCCGGCTCTAGGCTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	640-691 bp	1.0	83.8
rps4-trnT-UGU_p4	GATCATTTCCGGCTCTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.2	57.9	675-726 bp	1.0	83.3
rps4-trnT-UGU_p5	TCGAAGATCATTTCCGGCTC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	680-731 bp	1.0	83.1