Hotspot

trnT-GGU-psbD

LSC · 32223-33562 · 1340 bp

Back to Lasianthus

Pi 0.00752

MarkerSeek score 58.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1340
variable_sites: 15
pi: 0.00752
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1495-1508 bp	1.0	80.5
trnT-GGU-psbD_p2	GTGGCCCATTCAGGAATTCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1523-1536 bp	1.0	80.1
trnT-GGU-psbD_p3	AGTGGCCCATTCAGGAATTC	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1524-1537 bp	1.0	80.1
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1513-1526 bp	1.0	79.6
trnT-GGU-psbD_p5	GTGGCCCATTCAGGAATTCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1541-1554 bp	1.0	79.2