Hotspot

psbM-trnD-GUC

LSC · 30029-30920 · 892 bp

Back to Lasianthus

Pi 0.00299

MarkerSeek score 60.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 4
pi: 0.00299
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGTAGGAACTAAAATGAATAGTGCA	TTCAATTGGTCAGAGCACCG	36.0	50.0	57.5	58.5	1012-1028 bp	1.0	40.4
psbM-trnD-GUC_p2	AGGAACTAAAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	33.3	50.0	56.4	58.5	1009-1025 bp	1.0	40.4
psbM-trnD-GUC_p3	GGAACTAAAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	34.8	50.0	55.0	58.5	1008-1024 bp	1.0	40.4
psbM-trnD-GUC_p4	CGTAGGAACTAAAATGAATAGTGC	TTCAATTGGTCAGAGCACCG	37.5	50.0	55.9	58.5	1012-1028 bp	1.0	40.4
psbM-trnD-GUC_p5	CGTAGGAACTAAAATGAATAGTGCA	GTTCAATTGGTCAGAGCACC	36.0	50.0	57.5	57.0	1013-1029 bp	1.0	40.4